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The protein–ligand molecular interactions imply strong geometrical and structural rearrangements of the biological complex which are normally detected by high sensitivity optical techniques such as time-resolved fluorescence microscopy. In this work, we have measured, by optical spectroscopic reflectometry in the visible–near-infrared region, the interaction between a sugar binding protein (SBP), covalently bound on the surface of a porous silicon (PSi) microcavity, and glucose, at different concentrations and temperatures. Variable-angle spectroscopic ellipsometric (VASE) characterization of protein-functionalized PSi layers confirms that the protein–ligand system has an overall volume smaller than the SBP alone.
IOP Publishing
Publication date: 
15 Dec 2008
Biblio References: 
Volume: 21 Issue: 3 Pages: 035115
Journal of Physics: Condensed Matter