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Digital holography (DH) has been employed in the retrieval of three dimensional images of bull's sperm heads. The system allows a three dimensional analysis of the sperm morphology by means of a Digital Holographic Microscope (DHM). Microscopic holography measurements are performed by projecting a magnified image of a microscopic hologram plane onto a CCD plane. This could constitute the basis of an alternative method for the zoothecnic industry aimed at the investigation of morphological features and the sorting of the motile sperm cells. Indeed, one of the main advantages of digital holography consists in its full non-invasivity and in the capability of investigating the shape of the sperm cells without altering their characteristics. In particular the proposed technique could be applied to investigate the frequency of aberrant spermatozoa. Until now, in fact, such industrial investigations have been mainly …
International Society for Optics and Photonics
Publication date: 
17 Jun 2009

G Di Caprio, G Coppola, S Grilli, P Ferraro, R Puglisi, D Balduzzi, A Galli

Biblio References: 
Volume: 7389 Pages: 738907
Optical Measurement Systems for Industrial Inspection VI