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Porous biosilica nanoparticles obtained from diatomites (DNPs) have been recently demonstrated to be non‐toxic nanovectors of therapeutic agents in cancer cells. In this work, the internalization kinetics and intracellular spatial distribution of functionalized DNPs incubated with human lung epidermoid carcinoma cell line (H1355) up to 72 hours are investigated by Raman imaging. The label‐free Raman results are compared with confocal fluorescence microscopy and photoluminescence (PL) data. Raman bands specifically assigned to DNPs and cellular components provide evidence that the nanovectors are internalized and co‐localize with lipid environments. A considerable DNPs uptake in cells is observed within 6 hours, with equilibrium being achieved after 18 hours. The obtained data show the presence of DNPs up to 72 hours, without damage to cell viability or morphology. The PL measurements …
WILEY‐VCH Verlag GmbH & Co. KGaA
Publication date: 
16 Nov 2017

Stefano Managò, Nunzia Migliaccio, Monica Terracciano, Michela Napolitano, Nicola M Martucci, Luca De Stefano, Ivo Rendina, Anna Chiara De Luca, Annalisa Lamberti, Ilaria Rea

Biblio References: 
Journal of biophotonics