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The yeast Saccharomyces cerevisiae expresses one member of the metacaspase Cys protease family, encoded by the YCA1 gene. Combination of proteomics and metabolomics data showed that YCA1 deletion down-regulated glycolysis, the TCA cycle and alcoholic fermentation as compared with WT cells. Δyca1 cells also showed a down-regulation of the pentose phosphate pathway and accumulation of pyruvate, correlated with higher levels of certain amino acids found in these cells. Accordingly, there is a decrease in protein biosynthesis, and up-regulation of specific stress response proteins like Ahp1p, which possibly provides these cells with a better protection against stress. Moreover, in agreement with the down-regulation of protein biosynthesis machinery in Δyca1 cells, we have found that regulation of transcription, co-translational protein folding and protein targeting to different subcellular locations …
Royal Society of Chemistry
Publication date: 
1 Jan 2015

Maša Ždralević, Valentina Longo, Nicoletta Guaragnella, Sergio Giannattasio, Anna Maria Timperio, Lello Zolla

Biblio References: 
Volume: 11 Issue: 6 Pages: 1573-1583
Molecular BioSystems