-A A +A
A process to immobilize the enzyme glucose oxidase on SiO2 surfaces for the realization of integrated microbiosensors was developed. The sample characterization was performed by monitoring, step by step, oxide activation, silanization, linker molecule (glutaraldehyde) deposition, and enzyme immobilization by means of XPS, AFM, and contact angle measurements. The control of the environment during the procedure, to prevent silane polymerization, and the use of oxide activation to obtain a uniform enzyme layer are issues of crucial importance. The correct protocol application gives a uniform layer of the linker molecule and the maximum sample surface coverage. This result is fundamental for maximizing the enzyme bonding sites on the sample surface and achieving the maximum surface coverage. Thin SiO2 layers thermally grown on a Si substrate were used. The XPS Si 2p signal of the substrate was …
American Chemical Society
Publication date: 
4 Mar 2008

Sebania Libertino, Filippo Giannazzo, Venera Aiello, Antonino Scandurra, Fulvia Sinatra, Marcella Renis, Manuela Fichera

Biblio References: 
Volume: 24 Issue: 5 Pages: 1965-1972